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Pennsylvania is seen as crucial by both candidates. On the same day that Mr. Biden delivered his speech at the Democratic National Convention, Mr. Trump traveled to Scranton, Pa., where Mr. Biden grew up, for his own event. And Mr. Biden chose to go to Pittsburgh to deliver his speech on Monday rebuking and responding to Mr. Trump’s attempts to redirect the 2020 contest away from the virus and toward law and order.

A presentation on Wednesday by Priorities USA, a leading Democratic super PAC, showed Pennsylvania as the tipping point state of the 2020 race. The group showed findings from a private poll of battleground states that showed Mr. Biden with a wide lead on handling race relations but knotted in a virtual tie on the issue of policing and law enforcement.

Guy Cecil, the chairman of Priorities USA, who had previously predicted a tightening of the polls as the election neared, described the 2020 race as “just structurally close.”

“I don’t think this has to do with Kenosha,” he said. “I think it has to do with the fact that we’re two months before the election.”

In the new ad, Mr. Biden casts himself as a unifying figure who would seek to “lower the temperature” of the national debate and bring the country together. The spot ends with Mr. Biden, who is Catholic, quoting the former pope, John Paul II: “Be not afraid.”

The Biden campaign said the ad would air nationally on cable television and in local markets in nine battleground states: Arizona, Florida, Michigan, Minnesota, Nevada, North Carolina, Ohio, Pennsylvania and Wisconsin.

His speech in Pittsburgh and the advertising campaign are part of Mr. Biden’s response to a Republican convention last week in which the G.O.P. tried repeatedly to twist the former vice president’s record on crime and policing, with Mr. Trump himself often amplifying the misleading claims on Twitter.